Real-time Polymerase Chain Reaction:Article Title: TMPRSS2 is a functional receptor for human coronavirus HKU1
Article Snippet: .. Viral RNA was extracted from culture supernatants using the Quick-DNA/RNA Viral 96 Kit (Zymo) according to manufacturer’s instructions. qPCR was run using the Luna Universal Probe One-Step kit on a QuantStudio6 (Thermo) according to manufacturer’s instructions using the following primers (HKUqPCR5: 5’-CTGGTACGATTTTGCCTCAA-3’) and (HKUqPCR3: 5’-ATTATTGGGTCCACGTGATTG-3’)29 and TaqManTM QSY probe (5’FAM-TTGAAGGCTCAGGAAGGTCTGCTTCTAA-QSY7–3’). ..
Article Title: TMPRSS2 is a functional receptor for human coronavirus HKU1
Article Snippet: .. Analysis of HKU-1 replication by RT qPCR Viral RNA was extracted from culture supernatants using the Quick-DNA/RNA Viral 96 Kit (Zymo) according to manufacturer’s instructions. qPCR was run using the Luna Universal Probe One-Step kit on a QuantStudio6 (Thermo) according to manufacturer’s instructions using the following primers (HKUqPCR5: 5’-CTGGTACGATTTTGCCTCAA-3’) and (HKUqPCR3: 5’-ATTATTGGGTCCACGTGATTG-3’)29 and TaqManTM QSY probe (5’FAM-TTGAAGGCTCAGGAAGGTCTGCTTCTAA-QSY7–3’). ..
Lysis:Article Title: Evaluation of porcine epidemic diarrhea virus RNA contamination on swine industry transportation vehicles.
Article Snippet: .. The Quick-DNA/RNA Viral 96 Kit (Zymo Research Corporation) was used to isolate RNA according to the manufacturer’s instructions, with the following modifications: 1) Viral DNA/RNA Buffer was left on the sample for 10 min to allow for an adequate lysis period for environmental samples, 2) centrifuge steps were done at 3250 rpm for 10 min, 3) a five-minute dry spin at 3250 rpm was done before RNA elution, and 4) elution volume was increased to 18 μL. ..
Article Title: Evaluation of porcine epidemic diarrhea virus RNA contamination on swine industry transportation vehicles
Article Snippet: .. The Quick -DNA/RNA Viral 96 Kit (Zymo Research Corporation) was used to isolate RNA according to the manufacturer’s instructions, with the following modifications: 1) Viral DNA/RNA Buffer was left on the sample for 10 minutes to allow for an adequate lysis period for environmental samples, 2) centrifuge steps were done at 3,250 rpm for 10 minutes, 3) a five-minute dry spin at 3,250 rpm was done before RNA elution, and 4) elution volume was increased to 18 μL. ..
Environmental Sampling:Article Title: Evaluation of porcine epidemic diarrhea virus RNA contamination on swine industry transportation vehicles.
Article Snippet: .. The Quick-DNA/RNA Viral 96 Kit (Zymo Research Corporation) was used to isolate RNA according to the manufacturer’s instructions, with the following modifications: 1) Viral DNA/RNA Buffer was left on the sample for 10 min to allow for an adequate lysis period for environmental samples, 2) centrifuge steps were done at 3250 rpm for 10 min, 3) a five-minute dry spin at 3250 rpm was done before RNA elution, and 4) elution volume was increased to 18 μL. ..
Article Title: Evaluation of porcine epidemic diarrhea virus RNA contamination on swine industry transportation vehicles
Article Snippet: .. The Quick -DNA/RNA Viral 96 Kit (Zymo Research Corporation) was used to isolate RNA according to the manufacturer’s instructions, with the following modifications: 1) Viral DNA/RNA Buffer was left on the sample for 10 minutes to allow for an adequate lysis period for environmental samples, 2) centrifuge steps were done at 3,250 rpm for 10 minutes, 3) a five-minute dry spin at 3,250 rpm was done before RNA elution, and 4) elution volume was increased to 18 μL. ..
RNA Sequencing:Article Title: Interferon Regulatory Factor 6 Determines Intestinal Epithelial Cell Development and Immunity
Article Snippet: .. Bulk RNA sequencing and analysis RNA was extracted using the Zymo Quick-DNA/RNA Viral 96 Kit (ZymoResearch #D7023) from three M2C cell lines and three treatment groups each in triplicate experimental replicates (27 total samples). .. Quality of RNA samples were assessed using a TapeStation (Agilent) and mRNA sequencing libraries were prepared by the OHSU Massively Parallel Sequencing Shared Resource (MPSSR) using the TruSeq Stranded Poly(A)+ Library Prep Kit (Illumina).
Quantitative RT-PCR:Article Title: TMPRSS2 is a functional receptor for human coronavirus HKU1
Article Snippet: .. Analysis of HKU-1 replication by RT qPCR Viral RNA was extracted from culture supernatants using the Quick-DNA/RNA Viral 96 Kit (Zymo) according to manufacturer’s instructions. qPCR was run using the Luna Universal Probe One-Step kit on a QuantStudio6 (Thermo) according to manufacturer’s instructions using the following primers (HKUqPCR5: 5’-CTGGTACGATTTTGCCTCAA-3’) and (HKUqPCR3: 5’-ATTATTGGGTCCACGTGATTG-3’)29 and TaqManTM QSY probe (5’FAM-TTGAAGGCTCAGGAAGGTCTGCTTCTAA-QSY7–3’). ..
|